Multifocus confocal Raman microspectroscopy for rapid single-particle analysis.

Multifocus confocal Raman microspectroscopy for fast multimode vibrational imaging of living cells.

We have developed a multifocus confocal Raman microspectroscopic system for the fast multimode vibrational imaging of living cells. It consists of an inverted microscope equipped with a microlens array, a pinhole array, a fiber bundle, and a multichannel Raman spectrometer. Forty-eight Raman spectra from 48 foci under the microscope are simultaneously obtained by using multifocus excitation and...

Rapid identification of Candida species by confocal Raman microspectroscopy.

Candida species are important nosocomial pathogens associated with high mortality rates. Rapid detection and identification of Candida species can guide a clinician at an early stage to prescribe antifungal drugs or to adjust empirical therapy when resistant species are isolated. Confocal Raman microspectroscopy is highly suitable for the rapid identification of Candida species, since Raman spe...

Novel Method for Differentiating Histological Types of Gastric Adenocarcinoma by Using Confocal Raman Microspectroscopy

Gastric adenocarcinoma, a single heterogeneous disease with multiple epidemiological and histopathological characteristics, accounts for approximately 10% of cancers worldwide. It is categorized into four histological types: papillary adenocarcinoma (PAC), tubular adenocarcinoma (TAC), mucinous adenocarcinoma (MAC), and signet ring cell adenocarcinoma (SRC). Effective differentiation of the fou...

Raman microspectroscopy for non-invasive biochemical analysis of single cells.

Recent developments in biomedical vibrational spectroscopy now permit the non-invasive imaging of cells and tissues within both the laboratory and clinical settings. The rapid nature and diagnostic potential of both Raman and FTIR (Fourier-transform IR) spectroscopy have resulted in their widespread application to a number of biological fields including fundamental cell biology, medical imaging...

Confocal Raman Microspectroscopy; Applications in Cartilage Tissue Engineering